FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_las67.351000000b7219.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_las67.351000000b7219.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20732212
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT20558269.916095783701227TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAGCTCGTAT598570.2887149716585958TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACAACTCGTAT520650.2511309454099736TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACACCTCGTAT362780.17498374027817196TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2579700.066.5195646
TATGCCG2578750.066.4027348
GTATGCC2578850.066.39081647
CTCGTAT2588800.066.2415344
CGTCCGC2555200.066.2128633
TCGTATG2596650.066.2095245
ATGCCGT2537700.066.196849
GCCGTCT2530100.066.1949951
ACGTCCG2567850.066.1661932
TGCCGTC2540850.066.165150
CGCACAT2373550.066.0784137
GTCCGCA2564550.066.05546634
TCCGCAC2552250.066.05241435
CTGCTTG2605700.065.8366659
CCGCACA2556300.065.8255536
CACGTCC2572900.065.813631
CCGTCTT2538750.065.7969652
GTCACGT2596650.065.71557629
AGTCACG2597600.065.6871928
TCACGTC2592500.065.64986430
Produced by FastQC (version 0.11.2)