FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_las66.351000000b71fe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_las66.351000000b71fe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33697049
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1350721040.084251888051085TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAGCTCGTAT4851941.4398708919585212TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGAACTCGTAT4208731.2489906757116922TruSeq Adapter, Index 16 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGACCTCGTAT3086140.9158487439063284TruSeq Adapter, Index 16 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1802300.5348539570928007TruSeq Adapter, Index 16 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGCTCTCGTAT581030.1724275618318981TruSeq Adapter, Index 16 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA16576150.068.4499741
GCACACG16596200.067.8578911
CACGTCT16552950.067.79961414
ACGTCTG16534150.067.7943915
ACACGTC16586200.067.7763513
CACACGT16611500.067.76195512
CGTCTGA16556350.067.7584916
AGCACAC16641450.067.74972510
AGAGCAC16736950.067.666128
GAGCACA16711950.067.618869
TCTGAAC16611350.067.59383418
GAAGAGC16753850.067.570926
GAACTCC16538750.067.5697721
CTGAACT16612450.067.5479919
CGGAAGA16762200.067.524984
GTCTGAA16645750.067.5183717
ACTCCAG16462000.067.5040423
TCGGAAG16788850.067.499053
AAGAGCA16783850.067.4926767
TGAACTC16593400.067.4663620
Produced by FastQC (version 0.11.2)