FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_las64.351000000b71c7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_las64.351000000b71c7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22496276
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT227762610.12445793250403TruSeq Adapter, Index 14 (97% over 44bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG5732822.5483417788793132TruSeq Adapter, Index 14 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAGCTCGTAT646880.28754981491158804TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTAT546480.2429202059932053TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTACCTCGTAT355910.15820840747152995TruSeq Adapter, Index 14 (97% over 41bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3171600.059.658221
GCCGTCT3254900.053.6165351
ATGCCGT3343550.053.6103549
TGCCGTC3270800.053.60946350
CGTATGC3426250.053.50991446
CTCGTAT3428250.053.45467444
CGTATCT3172000.053.44750239
GTATGCC3427650.053.3808247
TATGCCG3428550.053.3614748
TCGTATG3441100.053.3538245
CCGTATC3194700.053.3419738
CCGTCTT3260950.053.27933552
GTATCTC3188850.053.2094940
TCCGTAT3209400.053.16807637
TCTCGTA3214750.053.1177543
TATCTCG3205550.053.0481941
GTTCCGT3449900.052.9947235
CTGCTTG3364150.052.9643559
ATCTCGT3232550.052.9547142
GTCACAG3478350.052.90401529