Basic Statistics
Measure | Value |
---|---|
Filename | HMYHGBBXX_l05n01_las64.351000000b71c7.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 22496276 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 39 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT | 2277626 | 10.12445793250403 | TruSeq Adapter, Index 14 (97% over 44bp) |
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG | 573282 | 2.5483417788793132 | TruSeq Adapter, Index 14 (97% over 43bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAGCTCGTAT | 64688 | 0.28754981491158804 | TruSeq Adapter, Index 14 (97% over 41bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTAACTCGTAT | 54648 | 0.2429202059932053 | TruSeq Adapter, Index 14 (97% over 41bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTACCTCGTAT | 35591 | 0.15820840747152995 | TruSeq Adapter, Index 14 (97% over 41bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 317160 | 0.0 | 59.65822 | 1 |
GCCGTCT | 325490 | 0.0 | 53.61653 | 51 |
ATGCCGT | 334355 | 0.0 | 53.61035 | 49 |
TGCCGTC | 327080 | 0.0 | 53.609463 | 50 |
CGTATGC | 342625 | 0.0 | 53.509914 | 46 |
CTCGTAT | 342825 | 0.0 | 53.454674 | 44 |
CGTATCT | 317200 | 0.0 | 53.447502 | 39 |
GTATGCC | 342765 | 0.0 | 53.38082 | 47 |
TATGCCG | 342855 | 0.0 | 53.36147 | 48 |
TCGTATG | 344110 | 0.0 | 53.35382 | 45 |
CCGTATC | 319470 | 0.0 | 53.34197 | 38 |
CCGTCTT | 326095 | 0.0 | 53.279335 | 52 |
GTATCTC | 318885 | 0.0 | 53.20949 | 40 |
TCCGTAT | 320940 | 0.0 | 53.168076 | 37 |
TCTCGTA | 321475 | 0.0 | 53.11775 | 43 |
TATCTCG | 320555 | 0.0 | 53.04819 | 41 |
GTTCCGT | 344990 | 0.0 | 52.99472 | 35 |
CTGCTTG | 336415 | 0.0 | 52.96435 | 59 |
ATCTCGT | 323255 | 0.0 | 52.95471 | 42 |
GTCACAG | 347835 | 0.0 | 52.904015 | 29 |