FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_las63.351000000b7191.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_las63.351000000b7191.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27817518
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT5674402.039865670258576TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC723850.061.23997546
TATGCCG724050.060.9473648
GTATGCC727950.060.6305647
ATGCCGT715150.060.57021749
TGCCGTC722250.059.8489850
GCCGTCT720400.059.83882551
CTCGTAT739450.059.81588444
TCGTATG743200.059.7516745
CCGTCTT749300.057.41192252
CTGCTTG776700.056.87062559
GTCACAG785200.056.58405729
CACAGTC789050.056.268331
CAGTCAC800950.055.6879727
TCTCGTA745650.055.58576243
CCAGTCA802200.055.58481226
AGTCAAC794850.055.49622734
ACAGTCA804600.055.14613332
AGTCACA812250.054.94077728
ACGTCTG827400.054.55254415
TCCAGTC817550.054.5449925
Produced by FastQC (version 0.11.2)