FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_las58.351000000b716a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_las58.351000000b716a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27682668
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1383610.4998109286287001TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC233550.049.7657444
TATGCCG236400.049.17723546
ATGCCGT237050.048.5550647
TGCCGTC239050.048.09025648
GTATGCC242700.048.02121445
GCCGTCT238150.047.4929449
TCGTATG252350.046.00613843
CTCGTAT253000.045.6383542
CCGTCTT266900.042.63052450
CTGCTTG279800.041.57298357
CACAGTG282350.040.9831133
GATCTCG261900.040.1639139
GTCACAC296750.039.0045729
CACACAG302000.038.31281731
AGTGATC276800.038.14790736
ACACAGT307250.037.8099132
TCTCGTA287200.036.76706741
CTTCTGC319450.036.03824654
TCTGCTT325750.035.8736256
ACAGTGA324450.035.8376834
Produced by FastQC (version 0.11.2)