FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_las57.351000000b7127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_las57.351000000b7127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23953629
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1117820.4666599787447655TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC182800.050.94628544
TATGCCG188100.049.3909746
ATGCCGT188050.049.01328747
GTATGCC191650.048.5224745
TGCCGTC188300.048.1115548
GCCGTCT189250.047.48160649
CTCGTAT200500.046.39883842
TCGTATG202050.046.11266343
ACTGACC224700.041.38482332
CTGACCA224200.041.32095733
CCGTCTT219950.041.1446850
CTGCTTG223350.040.95295357
CAGTCAC240900.039.25566527
CCAGTCA242250.038.93341426
GTCACTG245300.038.31041329
CACTGAC248700.037.68337231
TCCAGTC255500.036.92281725
ACTCCAG256200.036.86144623
ACGTCTG264350.036.27978515
TCTCGTA244700.036.1012241
Produced by FastQC (version 0.11.2)