FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_dj10.351000000b7325.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_dj10.351000000b7325.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35467726
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGATTCTCGATGCAAGAAGCTTACAGAGTGGTTTCAAAAACTGGACCAA1143221732.23273180806686No Hit
AGGATTCTCGATGCAAGAAGCTTCTTCGTTGCCCGAGCGACGAACAGCTT790723422.294166815205465No Hit
AGGATTCTCGATGCAAGAAGCTTGAATCCAAATTTTGCCAAAGTTTTGCT4671141.31701141482823No Hit
AGGATTCTCGATGCAAGAAGCTTTTGTTCCAACTAGTCTTTCCAAAGTAC3619451.0204911360824205No Hit
AGGATTCTCGATGCAAGAAGCTTTGCAACAAGCCACCGCCGGAATCACAG2457160.6927875782056059No Hit
AGGATTCTCGATGCAAGAAGCTTGTACAAGGCTGCTATGAACTTTGATGT930170.26225814420693333No Hit
AGGATTCTCGATGCAAGAAGCTTCTTGTACAAGGCTGCTATGAACTTTGA764980.21568340750123083No Hit
AGGATTCTCGATGCAAGAAGCTTCACGTAGTTACAGAAATAGTACAAGGC744620.20994297745505308No Hit
AGGATTCTCGATGCAAGAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG637160.17964501022704416No Hit
AGGATTCTCGATGCAAGAAGCTTTGAGCAGTTACAGAAAACCTACAGTAC571180.16104218240549167No Hit
AGGATTCTCGATGCAAGAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA537670.15159415633243586No Hit
AGGATTCTCGATGCAAGAAGCTTGTGTGAAAAAAGATCCACTGCGAGCAG524450.14786682405294324No Hit
AGGATTCTCGATGCAAGAAGCTTACAACATCCAGGATTCCCAGGCCGTCT469900.1324866443368825No Hit
AGGATTCTCGATGCAAGAAGCTTTGGAAGTTTCGGTTGTTGCATCTCTAC459910.12966999914231886No Hit
AGGATTCTCGATGCAAGAAGCTTTTCGCCACCGATGATACAAGGCGTTCG373370.10527035198140418No Hit
AGGATTCTCGATGCAAGAAGCTTGGAATTTGCAACAAAGTTTCTGAAAGA364280.10270745860617059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATTC34992150.069.918391
GGATTCT35010900.069.877812
GATTCTC35038950.069.8690263
ATTCTCG35054250.069.838084
CTCGATG35186950.069.818657
TCTCGAT35161350.069.787586
CGATGCA35172100.069.782759
TCGATGC35245000.069.7710658
GATGCAA35123150.069.76115410
ATGCAAG35125850.069.7569311
TTCTCGA35186100.069.71345
TGCAAGA35156650.069.6927412
GCAAGAA35167600.069.66585513
CTGGACC11995100.069.6651142
AGAGTGG11863600.069.6648426
GCGACGA8364250.069.6620237
AGCGACG8355250.069.6607536
GAGTGGT11855000.069.6604627
CAGAGTG11865750.069.65095525
GTAAGAG8348550.069.6472663
Produced by FastQC (version 0.11.2)