FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_dj09.351000000b72fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_dj09.351000000b72fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26917532
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTTGCGAAGACAACCTTACCACTTTCGATCGGAAGAGCACACGTCTG1664601461.84078837539786Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATT23449738.711693924985395No Hit
AAGCTTGCGAAGACAACGTTTTTCTCCAACCTTACCACTTTCGATCGGAA5737312.1314398363118876No Hit
AAGCTTGCGAAGACCACTTTCGATCGGAAGAGCACACGTCTGAACTCCAG3028771.1252034547595224Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AAGCTTGCGAAGACAACGTTTTTCTTCTCCAACCTTACCACTTTCGATCG3012271.11907362086539No Hit
AAGCTTGCGAAGACAACGTTTTTCGATCGGAAGAGCACACGTCTGAACTC2070520.7692087075442132Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
AAGCTTGCGAAGACAACTTTCGATCGGAAGAGCACACGTCTGAACTCCAG1691790.628508586894222Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AAGCTTGCGAAGACAACGTTTTTACGCCACGATCTACACTCTTCCCAACC843800.3134759902950984No Hit
AAGCTTGCGAAGACAACGTTTACCACTTTCGATCGGAAGAGCACACGTCT500900.1860868968224873Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAGCTTGCGAAGACAACGTTTTTTCTTCTCCAACCTTACCACTTTCGATC496820.18457115607775632No Hit
AAGCTTGCGAAGACAACGTTTTTACACTCTTCTCCAACCTTACCACTTTC430910.1600852559588301No Hit
AAGCTTGCGAAGACAACCTCACCACTTTCGATCGGAAGAGCACACGTCTG421610.15663025867304625Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCTTGCGAAGACAACGTTACCACTTTCGATCGGAAGAGCACACGTCTG417170.15498077609789782Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCTTGCGAAGACAACGTTTTTGATACGCACACCGTCCAACCTTACCAC408520.15176725711703434No Hit
AAGCTTGCGACAACCTTACCACTTTCGATCGGAAGAGCACACGTCTGAAC402510.14953451155923211Illumina Multiplexing PCR Primer 2.01 (96% over 25bp)
AAGCTTGCGAAGACAACCTTACCACTTTCGATCGAAAGAGCACACGTCTG320310.11899679361391675Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
AAGCTTGCGAAGACAACGTTTTAGACGACCACCGAGATCTACACTCTTCT307780.11434183490522087Illumina Single End PCR Primer 1 (96% over 25bp)
AAGCTTGCGAAGACAACGTTTTACACTCTTCTCCAACCTTACCACTTTCG300430.11161127253419817No Hit
AGCTTGCGAAGACAACCTTACCACTTTCGATCGGAAGAGCACACGTCTGA298980.11107259016168347Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AAGCTTGCGAAGACAACCTTACCACTTTCGATCGGAAGAGAACACGTCTG297200.11041131111128615Illumina Multiplexing Index Sequencing Primer (95% over 21bp)
AAGCTTGCGAAGACAACGTTTTTGATACGGCGACCACCGAGATCTACACT290580.10795194745194321Illumina Single End PCR Primer 1 (100% over 28bp)
AAGCTGCGAAGACAACCTTACCACTTTCGATCGGAAGAGCACACGTCTGA286220.10633218528355423Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
AAGCTTGCGAAGACAACGTTTTTTACACTCTTCTCCAACCTTACCACTTT274380.10193356508315846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCTTG26071450.069.989541
TAGGACG301.02763806E-769.9755422
AGCTTGC26104000.069.776432
GCTTGCG26110900.069.747853
TTGCGAA26168900.069.5860755
CTTGCGA26183700.069.5716554
TGCGAAG26210750.069.40066
GCGAAGA26274150.069.311447
CGAAGAC26342350.069.211828
GAAGACA25964100.069.133049
GCTGCGA43700.069.095743
AAGACAA25911000.069.0771510
AGACAAC25863200.069.04693611
AGCTGAC2543050.069.03982536
CTGAAGC2542550.069.0392332
AGCTGCG43700.069.017092
TGAAGCT2543900.069.0163633
GAAGCTG2541400.069.0154534
GACCAAA2522600.069.0096370
AGTATTA2520100.069.0062945
Produced by FastQC (version 0.11.2)