FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_dj08.351000000b72c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_dj08.351000000b72c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11806138
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGCCTAGTTTCGAAAGCTTTCAAGAAATCCGCGTTAGCGTAGTAAA184607415.636561253137987No Hit
TCAAAGCCTAGTTTCGAAAGCTTCTTGTACTAAAATTTTGTGGCGAAATT1086170.920004492578352No Hit
TCAAAGCCTAGTTTCGAAAGCTTCACGTAGTTACAGAAATAGTACTAAAA709290.6007807125412222No Hit
TCAAAGCCTAGTTTCGAAAGCTTACAACATCCAGGATTCCCAGGCCGTCT656930.5564309006044145No Hit
TCAAAGCCTAGTTTCGAAAGCTTTGAGCAGTTACAGAAAACCTACAGTAC649120.54981569756342No Hit
TCAAAGCCTAGTTTCGAAAGCTCATTGACCAGTGTTTGCAAGTGATCGGA601370.5093706341565718No Hit
TCAAAGCCTAGTTTCGAAAGCTTGTACTAAAATTTTGTGGCGAAATTTGA593070.5023403927685751No Hit
TCAAAGCCTAGTTTCGAAAGCTTAAAGTGGAGCATGAAAAAACAAAAACA546350.46276775690746624No Hit
TCAAAGCCTAGTTTCGAAAGCTTCGAATCTTAGATGCAGGCTTTCTGGTG543840.4606417441503733No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTCACAATGATGTTTAGTCCAGTTAGC514260.43558698026399484No Hit
TCAAAGCCTAGTTTCGAAAGCTTCATTGACCAGTGTTTGCAAGTGATCGG474070.4015453656394666No Hit
TCAAAGCCTAGTTTCGAAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG406260.3441091405165686No Hit
TCAAAGCCTAGTTTCGAAAGCTTCTTTTTCCTCTGTGTATAGAGCATCTG379730.3216377785860203No Hit
TCAAAGCCTAGTTTCGAAAGCTCTAAAATTTTGTGGCGAAATTTGAATAC363390.3077975202390485No Hit
TCAAAGCCTAGTTTCGAAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA340070.288045082989882No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTGCCCTTTTGCTCTACGAGAGGTTTC338050.2863341085797913No Hit
TCAAAGCCTAGTTTCGAAAGCTTGCTTGATCTTGACTTTCAGTACTAAAA309030.26175367423284396No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTCGCCACCGATGATACAAGGCGTTCG284510.2409848165420394No Hit
TCAAAGCCTAGTTTCGAAAGCTTTAACTTTAAATTGCAGTCGCTTTTCAC277840.23533521292060114No Hit
TCAAAGCCTAGTTTCGAAAGCTTCCATTGTAATTTTGTAAATCCAACTAA276780.23443737486382085No Hit
TCAAAGCCTAGTTTCGAAAGCTTGGAATTTGCAACAAAGTTTCTGAAAGA269950.22865224851683083No Hit
TCAAAGCCTAGTTTCGAAAGCTTGAGTGTTGTCATAGCACAATTGCACGT244540.20712954566514472No Hit
TCAAAGCCTAGTTTCGAAAGCTTGAGACCAGAAGTCAAATTGGCGGCATT238840.20230154856736385No Hit
TCAAAGCCTAGTTTCGAAAGCTTCACCTTCACCTCTGCGAGGAGCAACAA237390.20107337386705118No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTTCAGAATTTACCAACAACATCCCAA205000.17363849211317028No Hit
TCAAAGCCTAGTTTCGAAAGCTTTGGAAGTTTCGGTTGTTGCATCTCTAC167320.1417228902457349No Hit
TCAAAGCCTAGTTTCGAAAGCTTGAAATATAACTAAAAAATATAATAACA159340.13496369430884173No Hit
TCAAAGCCTAGTTTCGAAAGCTTCGGTACTAAAATTTTGTGGCGAAATTT154120.13054226538771613No Hit
TCAAAGCCTAGTTTCGAAAGCTTGACCAGTGTTTGCAAGTGATCGGAAGA147380.12483337057384897No Hit
TCAAAGCCTAGTTTCGAAAGCTTCACCCTTAGAATAAGCTCATTTAGTAT144430.1223346703214887No Hit
TCAAAGCCTAGTTTCGAAAGCTTGCTTTTAGTCCCCGCTTGTCGAAAGGG142980.12110649562117604No Hit
AAGCTTGCGAAGACAACCTTACCACTTTCGATCGGAAGAGCACACGTCTG142110.12036959080098844Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCAAAGCCTAGTTTCGAAAGCTTCCAAAACAAAGTGTTTGCAGCACACCA130490.11052725285779312No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTTGTACTAAAATTTTGTGGCGAAATT130050.11015456536252582No Hit
TCAAAGCCTAGTTTCGAAAGCTTCTGGCACCTATTGGGATGTTGTTGGTA125870.10661403415748655No Hit
TCAAAGCCTAGTTTCGAAAGCTTCTCTTCTCCAACAGTTTCCACAGCTTC121370.10280245750134379No Hit
TCAAAGCCTAGTTTCGAAAGCTTTACGTAGTTACAGAAATAGTACTAAAA121070.10254835239093428No Hit
TCAAAGCCTAGTTTCGAAAGCTTTGAGCAGTTACCGAAAACCTACAGTAC118900.10071032542563878No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGT11578550.069.8377466
TCAAAGC11603150.069.810521
CCTAGTT11604350.069.7901157
CAAAGCC11602600.069.7493742
AGCCTAG11621450.069.675315
CTAGTTT11614600.069.618578
GTTTCGA11572750.069.61790511
TAGTTTC11586350.069.613749
AAAGCCT11631250.069.611573
AGTTTCG11581650.069.51993610
GCGTTAG1949500.069.31837535
CGTTAGC1952250.069.29784436
CGCGTTA1951200.069.27856434
CGAAAGC11611200.069.2770515
CCGCGTT1948200.069.2483633
CGTAGTA1952550.069.1929642
GTTAGCG1953100.069.18072537
TTAGCGT1950200.069.1634638
AGCGTAG1951500.069.1292840
GAAAGCT11644050.069.11500516
Produced by FastQC (version 0.11.2)