FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l05n01_dj06.351000000b7290.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l05n01_dj06.351000000b7290.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6702195
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATT305822545.6302002552895No Hit
AAGCTTGCGAAGACAACCTTACCACTTTCGATCGGAAGAGCACACGTCTG210351931.385523697833325Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCTTGCGAAGACAACGTTTTTCTCCAACCTTACCACTTTCGATCGGAA1412682.1077870757266837No Hit
AAGCTTGCGAAGACAACGTTTTTCTTCTCCAACCTTACCACTTTCGATCG533950.7966792968572236No Hit
AAGCTTGCGAAGACCACGTTTTTTCACCATTCTGAAGCTGACAGAGTATT240570.35894210777215524No Hit
AAGCTTGCGAAGACAACGTCTGCTCCAACCTTACCACTTTCGATCGGAAG99170.1479664497974171No Hit
AAGCTTGCGAAGACAACGTCTGACTCCAACCTTACCACTTTCGATCGGAA99150.14793660882740653No Hit
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTTAAGCTGACAGAGTATT89150.13301612382212094No Hit
AAGCTTGCGAAGACCACTTTCGATCGGAAGAGCACACGTCTGAACTCCAG83040.12389970748389147Illumina Multiplexing PCR Primer 2.01 (96% over 30bp)
AAGCTTGCGAAGACAACGTTTTTACATCTTCTCCAACCTTACCACTTTCG76360.11393282350036071No Hit
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTAGT74650.11138142056445688No Hit
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTTACAGAGTATT73550.10974016721387546No Hit
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGAATT72070.1075319354330932No Hit
AAGCTTGCGAAGACAACGTTTACCACTTTCGATCGGAAGAGCACACGTCT71490.10666654730278663Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC68890.10278722120141237TruSeq Adapter, Index 22 (98% over 50bp)
AAGCTTGCGAAGACAACGTCTACTCCAACCTTACCACTTTCGATCGGAAG67040.10002693147543454No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG252.8376508E-670.02009631
TCATGCG150.002223303370.0164359
AAGCTTG6461300.070.010841
GCGATGT353.7161954E-970.003930
CCCGTGA207.930393E-570.0018128
TCAGACC2800.069.9986767
ACCCGTG207.932445E-569.9981527
CGTATGT301.0254371E-769.9960644
GTACCGC500.069.9934526
GGTCGTA252.8449558E-669.9897970
CGTAGCG353.7234713E-969.9850968
TAGCCCG1450.069.9840525
GGGCCAC252.8463437E-669.9840524
AGAGGCG1350.069.9840525
TCGGTGC150.002227782569.980928
TGCGCAG1550.069.980916
CAACCCG550.069.9788314
CGTCGCT1300.069.9788317
TAGTACG900.069.9788323
CCACGGA252.847857E-669.97777622
Produced by FastQC (version 0.11.2)