FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_las70.352000000b72b6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_las70.352000000b72b6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19186951
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5422872.826332333886713Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA621550.064.4321646
GCCGTAT621050.064.204347
GGTCGCC629650.064.02733643
TGGTCGC633050.063.6745642
GTGGTCG640400.063.0012441
GTCGCCG642600.062.73702644
CCGTATC638200.062.53559548
TCGCCGT639750.062.45337345
CGTATCA641050.062.27886249
CTCGGTG654700.061.64597737
CGGTGGT654550.061.54390739
GGTGGTC659000.061.19905540
ATCTCGG664250.060.95928635
TCGGTGG663950.060.81365238
GATCTCG668000.060.55946734
TCTCGGT669650.060.27636336
GTATCAT669350.059.83057850
AGATCTC693950.058.33614733
TAGATCT703850.057.52610432
GAGTGTA710000.056.68037827
Produced by FastQC (version 0.11.2)