FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_las65.352000000b7280.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_las65.352000000b7280.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35084358
Sequences flagged as poor quality0
Sequence length76
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3080397987.7997511027564Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAGAGTGTAGATCTCGGTGGTCGCCGT3136200.8939026331905517Illumina Single End PCR Primer 1 (96% over 30bp)
NATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2587710.7375680068023477Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1731630.49356183174279544Illumina Single End PCR Primer 1 (100% over 30bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGGTGTAGATCTCGGTGGTCGCCGT1408900.4015749696773702Illumina Single End PCR Primer 1 (96% over 27bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT900410.25664143547959467Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAAGTGTAGATCTCGGTGGTCGCCGT831540.23701160500072424Illumina Single End PCR Primer 1 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG529370.15088490432117924Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCGG505560.14409840419482664Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTAGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG474030.13511149327572133Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGTGTAGATCTCGGTGGTCGCCGTA437720.12476215183985981Illumina Single End PCR Primer 1 (96% over 30bp)
GATCAGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG424550.1210083422361612Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG408970.11656761682798927Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAGAGAGTGTAGATCTCGGTGGTCGCCG387480.11044238004868152Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCAG385350.1098352718895412Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGGCGCCG384760.10966710577973238Illumina Single End PCR Primer 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA33188900.070.4777451
ATCGGAA33579750.069.6517942
TCGGAAG33556400.069.63913
CGGAAGA33549700.069.604634
GAGCGTC33470100.069.512689
AGCGTCG33423550.069.5059610
AGAGCGT33562400.069.497658
GCGTCGT33341250.069.4936611
CGTCGTG33335100.069.4817612
GTCGTGT33310850.069.4783713
GAAGAGC33702850.069.477516
TCGTGTA33300850.069.4603914
GTGTAGG33429100.069.41772516
TGTAGGG33289050.069.407217
CGTGTAG33417500.069.3950715
GTAGGGA33345600.069.37440518
TAGGGAA33249650.069.34067519
AAGAGCG33753900.069.330467
AGGGAAA33024000.069.0857220
GGGAAAG32952400.069.08028421
Produced by FastQC (version 0.11.2)