FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_las62.352000000b725a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_las62.352000000b725a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13956272
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG11968098.575420427460857Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT352200.2523596559310395Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT1347700.064.728947
CGCCGTA1351100.064.6556646
GGTCGCC1352250.064.5663743
TGGTCGC1352850.064.5353342
CGTATCA1356350.064.35483649
CCGTATC1356600.064.3142848
GTGGTCG1360150.064.2191941
TCGCCGT1360700.063.9033945
GTCGCCG1366850.063.89206344
GTAGGGA1397300.063.8706418
CTCGGTG1370550.063.8076537
GGTGGTC1366700.063.80451240
CGGTGGT1364500.063.75055339
GTATCAT1375800.063.6477950
GAGTGTA1377700.063.60560627
ATCTCGG1382500.063.50583335
TGTAGGG1406500.063.4503717
GATCTCG1383300.063.44639234
TCGGTGG1378500.063.43457838
TCTCGGT1381200.063.3246136
Produced by FastQC (version 0.11.2)