FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_las61.352000000b7223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_las61.352000000b7223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22530091
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2648481.175530094396867Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT502950.22323478409385916Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA374850.051.90708546
GCCGTAT375850.051.65656347
GGTCGCC379500.051.24306543
TGGTCGC381600.050.86199642
GTGGTCG391150.049.6725541
GTCGCCG392400.049.63873344
CCGTATC395900.049.11281248
TCGCCGT395800.048.86332745
CGTATCA398200.048.7900849
CTCGGTG405450.048.00747337
CGGTGGT406250.047.6039339
GGTGGTC413550.046.78143340
GATCTCG422850.046.23206334
ATCTCGG423750.046.1633635
TCGGTGG425200.045.86834338
TCTCGGT427950.045.5415936
GTATCAT433450.045.08210850
AGATCTC445300.043.95314833
AGTGTAG454350.043.10698728
GAGTGTA454350.043.05306227
Produced by FastQC (version 0.11.2)