FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_las60.352000000b71ee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_las60.352000000b71ee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27576294
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5027081.8229715711618102Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT598300.062.74436647
CGCCGTA599900.062.72715846
GGTCGCC600750.062.4879143
TGGTCGC603300.062.09752742
GTGGTCG613750.061.15719241
GTCGCCG621350.060.45563544
CCGTATC624450.060.1685848
CGTATCA625200.060.09621449
TCGCCGT623350.060.01008645
CTCGGTG634550.059.36095437
CGGTGGT633750.059.0060839
GGTGGTC643900.058.17067740
ATCTCGG654900.057.7823735
GATCTCG659950.057.38304534
TCGGTGG658100.057.284738
TCTCGGT662150.056.91929236
GTATCAT671600.056.16491750
AGATCTC690300.054.80644233
GAGTGTA691950.054.5663627
AGTGTAG697500.054.14221628