Basic Statistics
Measure | Value |
---|---|
Filename | HMYHGBBXX_l04n02_las60.352000000b71ee.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 27576294 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 37 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 502708 | 1.8229715711618102 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCCGTAT | 59830 | 0.0 | 62.744366 | 47 |
CGCCGTA | 59990 | 0.0 | 62.727158 | 46 |
GGTCGCC | 60075 | 0.0 | 62.48791 | 43 |
TGGTCGC | 60330 | 0.0 | 62.097527 | 42 |
GTGGTCG | 61375 | 0.0 | 61.157192 | 41 |
GTCGCCG | 62135 | 0.0 | 60.455635 | 44 |
CCGTATC | 62445 | 0.0 | 60.16858 | 48 |
CGTATCA | 62520 | 0.0 | 60.096214 | 49 |
TCGCCGT | 62335 | 0.0 | 60.010086 | 45 |
CTCGGTG | 63455 | 0.0 | 59.360954 | 37 |
CGGTGGT | 63375 | 0.0 | 59.00608 | 39 |
GGTGGTC | 64390 | 0.0 | 58.170677 | 40 |
ATCTCGG | 65490 | 0.0 | 57.78237 | 35 |
GATCTCG | 65995 | 0.0 | 57.383045 | 34 |
TCGGTGG | 65810 | 0.0 | 57.2847 | 38 |
TCTCGGT | 66215 | 0.0 | 56.919292 | 36 |
GTATCAT | 67160 | 0.0 | 56.164917 | 50 |
AGATCTC | 69030 | 0.0 | 54.806442 | 33 |
GAGTGTA | 69195 | 0.0 | 54.56636 | 27 |
AGTGTAG | 69750 | 0.0 | 54.142216 | 28 |