FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_las59.352000000b71b7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_las59.352000000b71b7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36772981
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3872721.0531427952495882Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA498450.059.15867646
GGTCGCC501400.058.75457843
GCCGTAT500400.058.71050347
TGGTCGC508050.057.89058742
GTGGTCG519150.056.69118541
GTCGCCG522250.056.46250544
CCGTATC528150.055.67973348
TCGCCGT526750.055.59840445
CGTATCA534400.055.01712849
CTCGGTG548000.054.01558337
CGGTGGT552350.053.2617639
GGTGGTC554950.053.09235840
TCGGTGG577000.051.37962338
ATCTCGG588350.050.6482735
GATCTCG588350.050.56577734
TCTCGGT593950.049.8908136
GTATCAT592700.049.884550
AGATCTC638000.046.63356433
TAGATCT669700.044.4995232
ATCATTA688600.043.29876752