FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_las56.352000000b7181.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_las56.352000000b7181.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25168232
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3798881.5093948593608006Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT3683161.4634162622150018Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCGG47300.039.6523411
TGGTGCG53300.034.9260610
GGTCGCC887300.033.80544342
TGGTCGC887600.033.74916541
CGCCGTA867050.033.50175546
GCCGTAT864050.033.46294447
GTGGTCG898250.033.38924440
TGCGGAC55100.033.34950613
GTCGCCG902350.033.28334443
CTCGGTG922050.032.85491636
CGGTGGT914000.032.8028738
CCGTATC882300.032.7873648
GGTGGTC918050.032.63205339
CGTATCA887000.032.5907349
TCGCCGT884450.032.43332745
GATCTCG939750.032.36779433
TCGGTGG940850.032.24619737
ATCTCGG947100.032.18127434
TCTCGGT948950.031.99225235
AGATCTC968500.031.50508332