Basic Statistics
Measure | Value |
---|---|
Filename | HMYHGBBXX_l04n02_las56.352000000b7181.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 25168232 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 37 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG | 379888 | 1.5093948593608006 | Illumina Single End PCR Primer 1 (100% over 50bp) |
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT | 368316 | 1.4634162622150018 | Illumina Single End PCR Primer 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GGTGCGG | 4730 | 0.0 | 39.65234 | 11 |
TGGTGCG | 5330 | 0.0 | 34.92606 | 10 |
GGTCGCC | 88730 | 0.0 | 33.805443 | 42 |
TGGTCGC | 88760 | 0.0 | 33.749165 | 41 |
CGCCGTA | 86705 | 0.0 | 33.501755 | 46 |
GCCGTAT | 86405 | 0.0 | 33.462944 | 47 |
GTGGTCG | 89825 | 0.0 | 33.389244 | 40 |
TGCGGAC | 5510 | 0.0 | 33.349506 | 13 |
GTCGCCG | 90235 | 0.0 | 33.283344 | 43 |
CTCGGTG | 92205 | 0.0 | 32.854916 | 36 |
CGGTGGT | 91400 | 0.0 | 32.80287 | 38 |
CCGTATC | 88230 | 0.0 | 32.78736 | 48 |
GGTGGTC | 91805 | 0.0 | 32.632053 | 39 |
CGTATCA | 88700 | 0.0 | 32.59073 | 49 |
TCGCCGT | 88445 | 0.0 | 32.433327 | 45 |
GATCTCG | 93975 | 0.0 | 32.367794 | 33 |
TCGGTGG | 94085 | 0.0 | 32.246197 | 37 |
ATCTCGG | 94710 | 0.0 | 32.181274 | 34 |
TCTCGGT | 94895 | 0.0 | 31.992252 | 35 |
AGATCTC | 96850 | 0.0 | 31.505083 | 32 |