FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_las55.352000000b715b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_las55.352000000b715b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30237542
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3860351.2766745392201522Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT542280.1793399741288495Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA552650.055.1819246
GCCGTAT552200.054.98344447
GGTCGCC560850.054.0978143
TGGTCGC566300.053.07185742
CCGTATC574700.052.8865648
CGTATCA577500.052.65608249
GTCGCCG580050.052.3373144
GTGGTCG575750.052.33284841
TCGCCGT576400.052.2798545
GTAGGGA602200.051.68037818
CTCGGTG590250.051.5432237
CGGTGGT586400.051.26767339
GATCTCG602400.051.07798434
CGTGTAG615450.050.67923715
ATCTCGG609750.050.55888435
GGTGGTC597400.050.3634740
GTGTAGG626600.049.9351616
TCGGTGG612600.049.76556838
TGTAGGG627900.049.57066717
GTATCAT619700.049.2691950
Produced by FastQC (version 0.11.2)