FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_las54.352000000b7117.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_las54.352000000b7117.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28769858
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG26829439.325534383937523Illumina Single End PCR Primer 1 (100% over 50bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1103580.3835889631433009Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGA3170550.064.568618
CGTGTAG3204200.064.1856715
GTGTAGG3208200.064.1730916
TGTAGGG3197500.064.0856917
TAGGGAA3189450.063.9538719
CGCCGTA3143150.063.895546
GCCGTAT3143050.063.865147
GCGTCGT3222700.063.7019111
GGTCGCC3147800.063.66778643
AGCGTCG3234900.063.61635210
CGTCGTG3229500.063.60736512
CCGTATC3158300.063.57744248
TGGTCGC3143900.063.56731842
GATCGGA3249050.063.558091
GAGCGTC3240400.063.545059
CGTATCA3162050.063.5089249
GGGAAAG3193150.063.35757421
GTGGTCG3156750.063.32963641
TCGCCGT3162700.063.24313445
GTCGCCG3171400.063.21496644
Produced by FastQC (version 0.11.2)