FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_dj07.352000000b7365.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_dj07.352000000b7365.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32315749
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATC469529714.529438881333062No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTC26317898.1439826754441No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTT10628283.288885552366433No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAA8980162.778880353353407No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGT8005452.477259617284439No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAT7210582.231289765247279No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTG6925802.143165550642196No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATT6330321.9588962644808263No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTC6281641.9438324019659887No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAT5992371.85431877193996No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAG5875911.8182806160550389No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGA5804591.7962108815735633No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAA5746521.7782413150937644No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCT5410871.674375549828661No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAT5166621.5987932076090825No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCC5038781.559233548942344No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTT5016631.5523793058301079No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATA4729271.463456718889604No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACA4683681.4493490464974215No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAC4625621.4313825744840387No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGG4552631.4087960641110313No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCA4226591.3079040810720495No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGT4200751.2999079798521769No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTA3984111.2328694594081666No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAG3887861.2030852201507074No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTA3854251.1926847185253233No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAC3777951.169073939768501No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTG3652481.1302476696424395No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGA3532811.0932161900378667No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGT3445231.066114853163391No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAA3434931.062927552754541No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCC3313621.025388580657685No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGC3300391.0212946015888413No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAC3296161.0199856422947213No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAG3281521.015455343461171No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACT3224020.9976621615671046No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCA3211840.993893101471979No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCG3192510.9879114978891561No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATG3104460.9606647210931116No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCT3095480.9578858902512208No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACC2444730.7565134882066327No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAT2381270.7368760043284158No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGCA2243690.6943023353721431No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAA2208010.6832612791985728No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGC2205120.6823669784042449No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGA2180190.674652473628261No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCG1915480.5927388531208112No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACG1661700.5142074844064422No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGG1566320.4846924637272062No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGGT1518520.4699009142570082No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGC1486140.4598810320008365No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGG1469190.4546359114251073No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTT1450580.4488771094242625No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGGA1407630.4355863761659988No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAG1286740.39817737165863No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTG1131650.35018529200731197No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAC1082540.3349883674365709No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTA915170.2831962830259636No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGCT899400.2783163094873648No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTC881470.2727679312028324No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGGC779380.24117652355821922No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGGG596770.18466847232908018No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGCC531940.16460704655182215No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGCG440850.13641955196520433No Hit
NGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATC368610.114065126573424No Hit
AGAACATTGTGGTGCGGACAACAAACTAAGCTTCTTGCATCGAGAATCCT333460.10318807711992069No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACAT31177250.070.418971
GAACATT31397100.069.835582
AACATTG31323200.069.825563
TGGTGCG31274100.069.8163910
CATTGTG31380950.069.797735
GGTGCGG31320200.069.7911711
TGCGGAC31356800.069.7818813
GCGGACA31454700.069.779514
GTGGTGC31357400.069.7739
TGTGGTG31344250.069.7691968
GCAGCCT31308700.069.7643336
CTTGTAC31212600.069.76272641
TTGTACC8396100.069.75754542
ATTGTGG31426900.069.755846
GTGCGGA31355650.069.7558212
TTGTGGT31372550.069.754887
GGACAAC31549050.069.7497416
TTGTACA11622000.069.7492742
TGTACCT4212750.069.7485243
CAGCCTT31243350.069.7431437
Produced by FastQC (version 0.11.2)