FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_dj05.352000000b733f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_dj05.352000000b733f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13967111
Sequences flagged as poor quality0
Sequence length76
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACT1075486977.00138561224293No Hit
NTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAACT818240.5858333910283952No Hit
CTTGCAAACACTGGTCAATGAGCTTTCGAAACTAGGCTTTGAAGATCGGA665470.4764550091998267No Hit
CTTGCAAACACTGGTCAATGAGCTTTGGTATTTGAACTTTCAAAGAAACT580450.4155834374052014No Hit
CTTGCAAACACTGGTCAATGAAGCTTTCGAAACTAGGCTTTGAAGATCGG574590.4113878668251437No Hit
CTTGCAAACACTGGTCAATGAGAGTTGGTATTTGAACTTTCAAAGAAACT502600.3598453538459027No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGCACTTTCAAAGAAACT334780.23969165849687885No Hit
CTTGCAAACACTGGTCAATGCGATTTGGTATTTGAACTTTCAAAGAAACT282090.20196732166014864No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG241990.1732570178614604Illumina Single End PCR Primer 1 (100% over 50bp)
CTTGCAAACACTGGTCAATGAGAATTGGTATTTGAACTTTCAAAGAAACT217160.15547954047189858No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCAAAGAAAGT205230.1469380460998699No Hit
CTTGCAAACACTGGTCAATGGGATTTGGTATTTGAACTTTCAAAGAAACT193030.1382032404553812No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATGTGAACTTTCAAAGAAACT192390.13774502114288345No Hit
CTTGCAAACACTGGTCAATGAGATTTGGGATTTGAACTTTCAAAGAAACT190310.1362558083772657No Hit
CTTGCAAACACTGGTCAATGAGATGTGGTATTTGAACTTTCAAAGAAACT188620.1350458230052013No Hit
CTTGCAAACACTGATCAATGAGATTTGGTATTTGAACTTTCAAAGAAACT174910.12522990617028817No Hit
CTTGCAAACACTGGTCAATGAGACTTGGTATTTGAACTTTCAAAGAAACT170800.1222872790228416No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTAGTTGAACTTTCAAAGAAACT161160.11538535062834397No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTTCGAAGAAACT160520.1149271313158462No Hit
CTTGCAAACACTGGTCAATGAGATTTGATATTTGAACTTTCAAAGAAACT155620.11141888970453517No Hit
CTTGCAAACACTGGTCAATGAGGTTTGGTATTTGAACTTTCAAAGAAACT155620.11141888970453517No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGGACTTTCAAAGAAACT153250.10972204631294188No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGAACTTGCAAAGAAACT151450.1084333044965419No Hit
CTTGCAAACACTGGTCAATGAGATATGGTATTTGAACTTTCAAAGAAACT147320.10547635799557976No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTTAACTTTCAAAGAAACT144270.10329265658445758No Hit
CTTGCAAACACTGGTCAATGAGATTTGGTATTTGAAATTTCAAAGAAACT142320.10189651961669095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGCAA12867250.070.392811
AGGTCCG150.002226752269.9894812
CAGGGGC1100.069.97829
TCAGCCC150.002228207469.977955
TGCAAAC12887950.069.955633
GCAAACA12902400.069.948474
CAAACAC12956900.069.901265
AAACACT12931250.069.8593756
CACTGGT12870800.069.8376549
AACACTG12894500.069.8323447
ACACTGG12854650.069.829998
ACTGGTC12836650.069.8284110
CTGGTCA12863700.069.7944611
TTGCAAA12912700.069.793282
TGGTCAA12796450.069.74554412
GGTCAAT12747750.069.6970713
GTCAATG12823600.069.6326714
TCAATGA12673800.069.612315
CAATGAG12527900.069.6070516
AAAATCC12751650.069.5637770
Produced by FastQC (version 0.11.2)