FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_dj04.352000000b7315.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_dj04.352000000b7315.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40627747
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAT487295511.994155127528977No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATC39407179.69957059149748No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTC15343733.776662781719104No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTT11790402.902056075125209No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAA9333432.2973043521217162No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGT8707902.143338147694973No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTG7668131.8874120684073374No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAT7575991.8647329865473465No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAG7114971.7512588133425169No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATT6851231.686342587493222No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTC6844181.6846073202139416No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAT6482601.595609030449067No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAA6436301.5842128779624427No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCT6151361.514078543415169No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGA5953941.4654861368512508No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTT5624671.3844405401067403No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATA5602071.3788778393249324No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCC5329931.3118940609726648No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAAC5287081.3013470818354755No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCA5061401.2457988379222702No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGT4854581.1948927416526445No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACA4809591.1838190289016026No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGG4785481.1778846609436648No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAG4582811.1280000340653888No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAC4552811.1206159179833428No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTA4519361.1123826285518614No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTTA4481591.1030860264045654No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAA4249291.045908354209255No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCTG4109311.011454068570428No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACT4074541.0028958780313366No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGT3973660.9780655570194429No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGA3751550.9233960229200009No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCAG3721810.916075902510666No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTAC3719620.9155368620366765No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTCG3718260.9152021154409572No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACATG3664880.9020633115589698No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACTGC3635200.8947579593817988No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCA3510550.8640769570608973No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCC3447640.8485924656368468No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCT3026010.7448136368477435No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGA2654730.653427816216341No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACC2608750.6421104276345917No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGC2415010.594423805976738No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAT2284390.5622733645555094No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGCA2165910.5331110287754819No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACACG2124330.522876643885766No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCCG2113590.5202331303283936No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAA2022070.4977066535340983No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACAGG1786000.43960104408447753No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGC1741210.4285765587739827No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTT1636610.40283060736791537No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGGA1611860.39673871160022733No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACCGG1588370.3909569487079852No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAG1289690.3174406889951343No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGGT1289380.3173643864622865No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTG1141240.28090162124914286No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTC1106100.27225235994503955No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGAC1058810.26061253162770753No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGTA1056770.2601104117341284No Hit
AGAACATTGTGGTGCGGGCAACATCAAAGTTCATAGCAGCCTTGTACCAT833290.20510366966693971No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGCT805580.19828320777915642No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGGC737010.18140557978762642No Hit
AGAACATTGTGGTGCGGGCAACATCAAAGTTCATAGCAGCCTTGTACATC689330.16966975796122782No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGGG582610.14340199568536252No Hit
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGCC522410.12858453608072334No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG503340.12389069962456939Illumina Single End PCR Primer 1 (100% over 50bp)
AGAACATTGTGGTGCGGACAACATCAAAGTTCATAGCAGCCTTGTACGCG438110.10783516989017383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACAT38724950.070.416811
GAACATT38920450.069.792782
AACATTG38649450.069.756423
CATTGTG38665150.069.7068945
ACATTGT38629650.069.664284
ATTGTGG38701650.069.642086
GTGGTGC38700500.069.603879
TTGTGGT38746200.069.592977
TGGTGCG38619400.069.5900910
TGTGGTG38640950.069.586528
GGTGCGG38648700.069.5544611
TGCGGAC38081050.069.5136413
GCGGACA38194100.069.494314
GTGCGGA38056900.069.4905312
TTGTACA12105700.069.4736742
GCAGCCT38806750.069.4696936
CTTGTAC37727700.069.4693441
TGTACCA7207300.069.4571743
TTGTACC13002050.069.45608542
GTACCAT5552100.069.4439844
Produced by FastQC (version 0.11.2)