FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n02_dj03.352000000b72ed.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n02_dj03.352000000b72ed.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8893992
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG350574639.41701319272606No Hit
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG7074877.95466197855811Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGTGGGAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG4699925.284376239600845No Hit
AAAGTGGTAAGGTTGGAGAAAAACGTTGTCTTCGCAAGCTTAGATCGGAA1206121.3561064592817262No Hit
AAAGTGGTAAGGTTGGAGAAGAAAAACGTTGTCTTCGCAAGCTTAGATCG576680.6483927577177943No Hit
AAAGTGGTAAGGGTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG484910.5452107445115759No Hit
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAAATGTCG369060.41495427475086555No Hit
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGGCGTGTAG368440.41425717495585784Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGGCG354130.3981676619452772No Hit
AAAGTGGTAAGGTTGGAGAAGAATAAGAAAAAAATAACGGAAAGATGTCG292210.32854763080515476No Hit
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGGGTAG279930.3147405574459703Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGGGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG271020.304722558779005No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG263910.2967283982265781Illumina Single End PCR Primer 1 (100% over 50bp)
NAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG246740.2774232313228975No Hit
AAAGAGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG242070.2721724957701783No Hit
AAAGTGGGAAGGGTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG226450.254610078354017No Hit
AAAGTGGTAAAGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG209200.2352149630896902No Hit
AAAGTGGAAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG208190.23407936503653254No Hit
AAAGTGGTAAGGTTGGAGAAGAAAAAACGTTGTCTTCGCAAGCTTAGATC189450.21300896155517118No Hit
AAAGTGGCAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG188680.21214320858395194No Hit
AAAGTGGTAAGGATGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG171660.1930066948564829No Hit
AAAGTGGTAAGGTTGTAGAAGAATAAGAATAAAATAACGGAAAGATGTCG167440.18826191883239832No Hit
AAAGTGGTAAGGTTGGAGAAGAATAAAAATAAAATAACGGAAAGATGTCG158190.17786163963268686No Hit
AAAGTGGGAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGTAG156220.17564666125177536Illumina Single End PCR Primer 1 (100% over 22bp)
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTAG151060.1698449919900985No Hit
AAAGTGGTAAGGTTGTCTTCGCAAGCTTAGATCGGAAGAGCGTCGTGGAG137430.15452004004501016Illumina Single End PCR Primer 1 (95% over 22bp)
AAAGTGGTAAGGCTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG135490.15233879229934094No Hit
AAAGTGGGAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAAATGTCG119390.13423668471930264No Hit
AAAGCGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG118140.1328312415842065No Hit
AAAGTGGTAAGGTTGGGGAAGAATAAGAATAAAATAACGGAAAGATGTCG117530.1321453853342796No Hit
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTGG116910.1314482855392719No Hit
AAAGTGGTAAGGTTGGCGAAGAATAAGAATAAAATAACGGAAAGATGTCG107650.12103676279447968No Hit
AAAGTGGTAAGGTTGGAGACGAATAAGAATAAAATAACGGAAAGATGTCG103210.1160446287786182No Hit
AAAGTGGTACGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG101260.11385213748786821No Hit
AAAGTGGTAAGGTTGGAAAAGAATAAGAATAAAATAACGGAAAGATGTCG97980.11016425470137595No Hit
ACAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTCG96440.10843274875893749No Hit
AAAGTGGTAAGGTTGGAGAAGAGTGTAAAAACGTTGTCTTCGCAAGCTTA95140.10697108789843751No Hit
AAAGTGGTAAGGTTGGAGAAGAATAAGAATAAAATAACGGAAAGATGTTG95070.10689238308287212No Hit
AAAGTGGTAAGGTTGGAGAAGAATAAGAAAACGTTGTCTTCGCAAGCTTA94410.10615030910754136No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGTGG7316950.070.47151
AAGTGGT6388850.069.8424452
AGTGGTA6429750.069.827563
GTGGTAA6383600.069.796494
TGGTAAG6283850.069.71495
AGTGGGA829850.069.6222843
GGTAAGG6533900.069.610996
GTAAGGT6365050.069.55117
TAAGGTT6313000.069.5336768
AAGGTTG7130250.069.487179
GGTTGGA5996450.069.3830311
AGGTTGG6079950.069.3711710
AGGTTGT1023550.069.3402810
GTTGGAG5995550.069.3062912
TTGGAGA5903300.069.3060913
AGCGGTA93400.069.294073
TAACGGA5878900.069.26559435
ATAACGG5920300.069.2552134
AAGTGGG826650.069.22912
AATAACG5939750.069.2113133
Produced by FastQC (version 0.11.2)