FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n01_las70.351000000b72b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_las70.351000000b72b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19186951
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT4680212.43926718737125TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC634550.063.57306346
TATGCCG636800.063.26561748
ATGCCGT629500.063.22622749
GTATGCC639800.063.0021747
CGTCCGC631300.062.93749233
TGCCGTC631600.062.9341950
GCCGTCT629100.062.88432751
TCGTATG646900.062.5755345
CTCGTAT646900.062.54307644
GTCCGCA635250.062.53254734
TCCGCAC634950.062.455435
CGCACAT585700.062.3225337
ACGTCCG641400.062.22931732
CCGCACA641300.061.8047236
CACGTCC654600.060.82211731
CCGTCTT649700.060.7350152
GCACATC610050.060.20310638
CTGCTTG674300.059.8741359
TCTCGTA627350.059.31141343
CATCTCG625400.059.17116541
Produced by FastQC (version 0.11.2)