FastQCFastQC Report
Wed 10 Jan 2018
HMYHGBBXX_l04n01_las65.351000000b7283.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_las65.351000000b7283.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35084358
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT2753873578.49291413569546TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAGCTCGTAT8820222.5140035339965463TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAAACTCGTAT7385062.1049437472961596TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAACCTCGTAT5609211.5987780081368455TruSeq Adapter, Index 15 (97% over 40bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCC1817060.5179117143885033TruSeq Adapter, Index 15 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACATGTCAGAATCTCGTAT1526220.4350143730718971TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACATGTCAGAATCTCGTAT1462480.4168467326664493TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACATGTCAGAATCTCGTAT1435810.40924505444848097TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1412180.40250985923698535TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1404220.40024104189109005TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACATGTCAGAATCTCGTAT1332140.3796962737639378TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1331630.3795509098385098TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACATGTCAGAATCTCGTAT1291410.3680871116410339TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATATGTCAGAATCTCGTAT1272860.3627998551377226TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTTAGAATCTCGTAT1177350.3355768972600268TruSeq Adapter, Index 15 (97% over 40bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1165870.33230478380137385TruSeq Adapter, Index 15 (97% over 40bp)
GAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGT955830.27243764870943343TruSeq Adapter, Index 15 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG954880.27216687276991075TruSeq Adapter, Index 15 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACATGTCAGAATCTCGTA600530.17116744732795167Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT496820.141607265551218TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCATTCACATGTCAGAATCTCGTAT450740.1284732073478443TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCGGTCACATGTCAGAATCTCGTAT433210.12347667869538899TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTTCAGAATCTCGTAT408280.11637094798770439TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATATCGTAT398050.11345511866000227TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGACTCCAGTCACATGTCAGAATCTCGTATG352820.10056333366567516Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA33433400.069.9633941
ATCGGAA33606850.069.565882
TCGGAAG33563250.069.5411453
CGGAAGA33693650.069.08044
GGAAGAG33804050.068.981395
GAAGAGC33728250.068.753326
AAGAGCA33798850.068.722867
AGAGCAC33630350.068.691568
GAGCACA33574850.068.609919
GATTGGA147550.068.564751
GTATGAC77650.068.5094647
AGCACAC33450800.068.5043610
GCACACG33430750.068.4752111
CACACGT33422850.068.3952812
CACGTCT33389150.068.34553514
ACACGTC33432100.068.3417213
AGAGTAC178550.068.3413548
GTACACG176750.068.3003111
ACGTCTG33540600.068.2879315
CGTCTGA33609900.068.25767516
Produced by FastQC (version 0.11.2)