FastQCFastQC Report
Tue 9 Jan 2018
HMYHGBBXX_l04n01_las62.351000000b725d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_las62.351000000b725d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13956272
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC11266058.072392111589686TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAGCTCGTATGC370450.2654362139115661TruSeq Adapter, Index 9 (98% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC315360.2259629219034997TruSeq Adapter, Index 9 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGAACTCGTATGC298420.2138250100026712TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGACCTCGTATGC228730.16389047161018358TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1361300.065.3172844
GTATGCC1357000.065.2895945
ATGCCGT1336150.065.268747
TATGCCG1354250.065.236346
GCCGTCT1319800.065.0783449
TCGTATG1364450.065.00584443
TGCCGTC1329000.064.9836148
AGTCACG1373350.064.8535528
GATCTCG1250450.064.7613439
CTCGTAT1356950.064.7435642
GTCACGA1380350.064.6088129
CACGATC1378850.064.5874631
CTGCTTG1357050.064.4489157
CGATCAG1381200.064.3941933
CAGATCT1258150.064.2908137
CCGTCTT1329300.064.2378750
CAGTCAC1388350.064.2009727
AGATCTC1261700.064.1256338
ACGATCA1391950.063.9261232
CCAGTCA1394450.063.88499526
Produced by FastQC (version 0.11.2)