FastQCFastQC Report
Tue 9 Jan 2018
HMYHGBBXX_l04n01_las61.351000000b7226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_las61.351000000b7226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22530091
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC2443621.0846028096380083TruSeq Adapter, Index 8 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC466150.20690107288070872TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC380950.050.65937444
TATGCCG382150.050.2073746
ATGCCGT378400.050.0019847
GCCGTCT371850.049.81350349
GTATGCC388050.049.5701645
TGCCGTC378100.049.42551448
TCGTATG393500.048.9515143
CTCGTAT390950.048.82331542
CCGTCTT395850.046.52028350
GTCACAC429100.045.83533529
CTGCTTG412400.045.78828457
TCTCGTA394850.044.78635841
CAGTCAC455200.043.7430927
ATCTCGT412100.043.05812540
AGTCACA464100.042.9290528
CCAGTCA468950.042.4905526
AATCTCG418900.042.28114739
TGCTTGA445250.042.2724358
CTTCTGC442900.042.19442754
TCTGCTT450250.041.77438756
Produced by FastQC (version 0.11.2)