FastQCFastQC Report
Tue 9 Jan 2018
HMYHGBBXX_l04n01_las60.351000000b71e1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_las60.351000000b71e1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27576294
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC4626181.6775930804915264TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC609800.062.23312444
ATGCCGT602450.061.6376847
TATGCCG612350.061.5842246
GTATGCC617600.061.2318445
TGCCGTC605150.061.16479548
GCCGTCT602000.061.11810749
TCGTATG626500.060.36263743
CTCGTAT628250.059.83241742
CCGTCTT625800.058.4261650
AGTCACC656550.057.4768228
CTGCTTG652400.057.46911657
CATCTCG620600.056.6324239
TCTCGTA628700.056.131741
GTCACCA679750.055.56682229
ACCAGAT678350.055.5453532
ATCTCGT642050.054.9716140
CAGTCAC690600.054.90807327
CCAGATC689150.054.4160233
TCACCAG698150.054.14136530
CTTCTGC688950.053.8529454
Produced by FastQC (version 0.11.2)