FastQCFastQC Report
Tue 9 Jan 2018
HMYHGBBXX_l04n01_las59.351000000b71ba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_las59.351000000b71ba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36772981
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC3803771.0343926156000243TruSeq Adapter, Index 6 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC515050.058.83635344
TATGCCG516850.058.26236746
ATGCCGT514000.057.84322747
GTATGCC523400.057.7105145
GCCGTCT516050.057.163449
TGCCGTC519350.057.00948748
TCGTATG534150.056.5752643
CTCGTAT538750.055.83236342
CCGTCTT556500.052.77039350
CTGCTTG578950.052.0278157
TATCTCG552050.051.093239
TCTCGTA563900.050.21956341
CACGCCA597600.050.11018831
CGCCAAT608950.049.06813433
GCCAATA611050.048.96463434
TCACGCC614600.048.85736530
ACGCCAA619600.048.46852532
GTCACGC624100.048.2719429
ATCTCGT588450.048.13853540
TGCTTGA634950.047.38849358
Produced by FastQC (version 0.11.2)