FastQCFastQC Report
Tue 9 Jan 2018
HMYHGBBXX_l04n01_las56.351000000b7184.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_las56.351000000b7184.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25168232
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC3696921.4688834718306791TruSeq Adapter, Index 3 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC3566891.417219135615088TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGCATC830350.033.3842236
CGTATGC906100.033.2855643
TATGCCG906950.033.2122845
GTATGCC911550.033.17519844
ACTTAGG914950.033.13123331
CACTTAG914900.033.11569230
GGCATCT835750.033.09530637
TGCCGTC878400.032.930947
ATGCCGT882300.032.89640846
GCCGTCT870750.032.7523649
CTCGTAT915700.032.6954341
TCGTATG920500.032.662242
GCATCTC862450.032.11962538
CATCTCG865000.032.09302539
CTGCTTG935400.031.8748956
TTAGGCA934200.031.8351833
TCTCGTA876100.031.75258641
CCGTCTT894450.031.72086750
ATCTCGT884300.031.4511740
TAGGCAT887350.031.27524635
Produced by FastQC (version 0.11.2)