FastQCFastQC Report
Tue 9 Jan 2018
HMYHGBBXX_l04n01_las55.351000000b715e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_las55.351000000b715e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30237542
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC3836271.268710928950508TruSeq Adapter, Index 2 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC525260.1737112097272986TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC570700.054.44917744
GTATGCC575600.053.79719545
ATGCCGT569150.053.438247
GTCACCG587450.053.34421529
TATGCCG581400.053.1550446
GCCGTCT551800.053.029149
TCGTATG584750.053.0028143
CTCGTAT582250.052.8457942
TGCCGTC560050.052.66675648
AGTCACC595450.052.65086428
CGATGTA584150.052.58448434
CCGATGT592050.052.09340733
GTATCTC553250.051.46380238
CAGTCAC620200.050.86925527
CCAGTCA624450.050.56227526
CCGTCTT574950.050.44458850
ACCGATG618000.050.252832
ACTCCAG633850.049.9464223
TATCTCG571850.049.8498639
TCCAGTC633750.049.8313425
Produced by FastQC (version 0.11.2)