FastQCFastQC Report
Tue 9 Jan 2018
HMYHGBBXX_l04n01_las54.351000000b711a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_las54.351000000b711a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28769858
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC25870368.992175074343432TruSeq Adapter, Index 1 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1035270.3598453631575102TruSeq Adapter, Index 1 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAACTCGTATGC610500.21220125591165587TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC598850.20815187895609358TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGACCTCGTATGC541800.18832209738400518TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3176900.064.42441644
CACGATC2923300.064.37784636
GTATGCC3166350.064.3616745
TATGCCG3159450.064.3180346
GATCTCG2931700.064.2228239
ATGCCGT3118950.064.19041447
ACGATCT2925450.064.1831637
GCCGTCT3014100.064.1663349
CGATCTC2930550.064.16563438
CTCGTAT3151800.064.1384442
TCGTATG3184350.064.1091743
TGCCGTC3042550.064.067248
CATCACG3204750.063.92263433
CAGTCAC3228150.063.92215327
TCACGAT2959150.063.86828235
CCAGTCA3231150.063.85630826
ACTCCAG3241850.063.77832423
ATCACGA3189350.063.76272234
GAACTCC3267200.063.73121321
TCCAGTC3239600.063.7102725
Produced by FastQC (version 0.11.2)