FastQCFastQC Report
Tue 9 Jan 2018
HMYHGBBXX_l04n01_dj07.351000000b7368.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_dj07.351000000b7368.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32315749
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGATTCTCGATGCAAGAAGCTTACAGAGTGGTTTCAAAAACTGGACCAA622362019.258783078182713No Hit
AGGATTCTCGATGCAAGAAGCTTCTTCGTTGCCCGAGCGACGAACAGCTT23914177.400159594010958No Hit
AGGATTCTCGATGCAAGAAGCTTCACGTAGTTACAGAAATAGTACAAGGC1805730.5587770842012667No Hit
AGGATTCTCGATGCAAGAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG1674570.5181900626842968No Hit
AGGATTCTCGATGCAAGAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA1355350.41940850574127186No Hit
AGGATTCTCGATGCAAGAAGCTTTGAGCAGTTACAGAAAACCTACAGTAC1232660.3814424972789583No Hit
AGGATTCTCGATGCAAGAAGCTTTGGAAGTTTCGGTTGTTGCATCTCTAC1173500.3631356339597761No Hit
AGGATTCTCGATGCAAGAAGCTTGTACAAGGCTGCTATGAACTTTGATGT1101430.34083381449707384No Hit
AGGATTCTCGATGCAAGAAGCTTTTCGCCACCGATGATACAAGGCGTTCG1014120.3138160282158399No Hit
AGGATTCTCGATGCAAGAAGCTTACAACATCCAGGATTCCCAGGCCGTCT890010.2754106055224033No Hit
AGGATTCTCGATGCAAGAAGCTTCTTGTACAAGGCTGCTATGAACTTTGA874530.27062037150987894No Hit
AGGATTCTCGATGCAAGAAGCTTCACCCTTAGAATAAGCTCATTTAGTAT866010.2679838861231408No Hit
AGGATTCTCGATGCAAGAAGCTTGAATCCAAATTTTGCCAAAGTTTTGCT841360.2603560264068148No Hit
AGGATTCTCGATGCAAGAAGCTTTGCAACAAGCCACCGCCGGAATCACAG679900.21039277164827588No Hit
AGGATTCTCGATGCAAGAAGCTTGCTTTTAGTCCCCGCTTGTCGAAAGGG573960.17760999443336437No Hit
AGGATTCTCGATGCAAGAAGCTTCTGGCACCTATTGGGATGTTGTTGGTA549490.1700378351125329No Hit
AGGATTCTCGATGCAAGAAGCTTTTGTTCCAACTAGTCTTTCCAAAGTAC532180.16468131374581477No Hit
AGGATTCTCGATGCAAGAAGCTTGAGTGTTGTCATAGCACAATTGCACGT454500.14064349862353492No Hit
AGGATTCTCGATGCAAGAAGCTTTTCGAGGAGGGAGAATACACTCACCCA445120.1377408891249898No Hit
AGGATTCTCGATGCAAGAAGCTTGCTTGATCTTGACTTTCAGTACAAGGC418820.12960244244996458No Hit
AGGATTCTCGATGCAAGAAGCTTTTGCCCTTTTGCTCTACGAGAGGTTTC416500.1288845262413692No Hit
AGGATTCTCGATGCAAGAAGCTTTTTCAGAATTTACCAACAACATCCCAA352810.10917586963557614No Hit
AGGATTCTCGATGCAAGAAGCTTGGAATTTGCAACAAAGTTTCTGAAAGA343130.10618042614454024No Hit
AGGATTCTCGATGCAAGAAGCTTCTGGTAATGTTCCCATAATTGGGGTAA328200.10156038778491566No Hit
AGGATTCTCGATGCAAGAAGCTTAGTTTGTTGTCCGCACCACAATGTTCT327550.10135924746785228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTCT32114300.069.81442
CTCGATG32135300.069.772127
AGGATTC32145500.069.7618261
TCGATGC32132850.069.75868
GATTCTC32136200.069.754063
CGATGCA32061050.069.729159
ATTCTCG32105450.069.726374
ATGCAAG32093700.069.72378511
GATGCAA32067050.069.7141810
TCTCGAT32124450.069.673476
TGCAAGA32082500.069.6484312
GCAAGAA32105700.069.59334613
TTCTCGA32170050.069.579425
CTGGACC6618300.069.5147342
CAAGAAG32161100.069.4818214
AGAGTGG6518800.069.4613626
GAAGCTT32250850.069.458617
TACAGAG6647300.069.4428623
AAGAAGC32199150.069.4266715
GACTTAC6636500.069.4128955
Produced by FastQC (version 0.11.2)