FastQCFastQC Report
Tue 9 Jan 2018
HMYHGBBXX_l04n01_dj05.351000000b7332.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_dj05.351000000b7332.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13967111
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGCCTAGTTTCGAAAGCTTTCAAGAAATCCGCGTTAGCGTAGTAAA6548974.688850829638284No Hit
TCAAAGCCTAGTTTCGAAAGCTTCTTGTACTAAAATTTTGTGGCGAAATT2366331.6942157902231894No Hit
TCAAAGCCTAGTTTCGAAAGCTTCACGTAGTTACAGAAATAGTACTAAAA1534081.0983516920571477No Hit
TCAAAGCCTAGTTTCGAAAGCTTACAACATCCAGGATTCCCAGGCCGTCT1089550.7800825811436596No Hit
TCAAAGCCTAGTTTCGAAAGCTTTGAGCAGTTACAGAAAACCTACAGTAC807040.5778145530596843No Hit
TCAAAGCCTAGTTTCGAAAGCTCATTGACCAGTGTTTGCAAGTGATCGGA668600.478695988025011No Hit
TCAAAGCCTAGTTTCGAAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG661730.47377729009241787No Hit
TCAAAGCCTAGTTTCGAAAGCTTGTACTAAAATTTTGTGGCGAAATTTGA644430.4613910493014625No Hit
TCAAAGCCTAGTTTCGAAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA641860.45955101237471374No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTCGCCACCGATGATACAAGGCGTTCG615900.440964491511523No Hit
TCAAAGCCTAGTTTCGAAAGCTTCATTGACCAGTGTTTGCAAGTGATCGG577700.41361452629681256No Hit
TCAAAGCCTAGTTTCGAAAGCTTGCTTGATCTTGACTTTCAGTACTAAAA567480.4062973366503638No Hit
TCAAAGCCTAGTTTCGAAAGCTTGGAATTTGCAACAAAGTTTCTGAAAGA325590.23311191555648123No Hit
TCAAAGCCTAGTTTCGAAAGCTTGCTTTTAGTCCCCGCTTGTCGAAAGGG315700.22603099524303918No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTGCCCTTTTGCTCTACGAGAGGTTTC309840.22183542466298148No Hit
TCAAAGCCTAGTTTCGAAAGCTTGAGTGTTGTCATAGCACAATTGCACGT253420.1814405283956002No Hit
TCAAAGCCTAGTTTCGAAAGCTTTGGAAGTTTCGGTTGTTGCATCTCTAC223050.1596965900822296No Hit
TCAAAGCCTAGTTTCGAAAGCTTCACGTAGTTACAAAAATAGTACTAAAA198160.14187615463212114No Hit
TCAAAGCCTAGTTTCGAAAGCTTTACGTAGTTACAGAAATAGTACTAAAA189780.1358763455091035No Hit
TCAAAGCCTAGTTTCGAAAGCTTCAAGTAGTTACAGAAATAGTACTAAAA177480.12706994309703704No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATCTCGTATGC167190.11970263571328386TruSeq Adapter, Index 25 (100% over 50bp)
TCAAAGCCTAGTTTCGAAAGCTTCTGGCACCTATTGGGATGTTGTTGGTA161090.11533523289103953No Hit
TCAAAGCCTAGTTTCGAAAGCTTCACCCTTAGAATAAGCTCATTTAGTAT146320.10476039031980199No Hit
TCAAAGCCTAGTTTCGAAAGCTTTTTCAGAATTTACCAACAACATCCCAA143860.1029991098373887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTAGT13703250.069.762616
CCTAGTT13735500.069.756267
TCAAAGC13813450.069.728741
CAAAGCC13804300.069.7061542
CTAGTTT13710500.069.650178
AGCCTAG13772450.069.5867165
GTTTCGA13699500.069.5225211
TAGTTTC13688950.069.516949
AAAGCCT13840000.069.48473
AGTTTCG13693950.069.4525710
CGAAAGC13749250.069.2386315
GAAAGCT13811250.069.07503516
AAAGCTT13724950.069.0397417
TCGAAAG13811250.068.9214814
CGCGTTA692350.068.8415134
TTTCGAA13824350.068.8235212
CGTTAGC693150.068.7898936
GCGTTAG693100.068.7688735
AAGCTTC4080450.068.6070118
ATCCGCG698500.068.5803931
Produced by FastQC (version 0.11.2)