FastQCFastQC Report
Tue 9 Jan 2018
HMYHGBBXX_l04n01_dj04.351000000b7318.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_dj04.351000000b7318.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40627747
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGATTCTCGATGCAAGAAGCTTACAGAGTGGTTTCAAAAACTGGACCAA502700612.373331949714071No Hit
AGGATTCTCGATGCAAGAAGCTTTTCGAGGAGGGAGAATACACTCACCCA490016812.061136444509216No Hit
AGGATTCTCGATGCAAGAAGCTTCTTCGTTGCCCGAGCGACGAACAGCTT12133322.986461444687051No Hit
AGGATTCTCGATGCAAGAAGCTTGTCTCCAAGCAACATCAACTGTCTCCG1981250.48765933291846086No Hit
AGGATTCTCGATGCAAGAAGCTTCAGACTCAAGCGCCTCGACGCGAATCA1719120.42313938796556944No Hit
AGGATTCTCGATGCAAGAAGCTTCACGTAGTTACAGAAATAGTACAAGGC1560260.384038031939108No Hit
AGGATTCTCGATGCAAGAAGCTTGTACAAGGCTGCTATGAACTTTGATGT1549880.38148312777472004No Hit
AGGATTCTCGATGCAAGAAGCTTCTTGTACAAGGCTGCTATGAACTTTGA1378730.3393567455266471No Hit
AGGATTCTCGATGCAAGAAGCTTTTCGCCACCGATGATACAAGGCGTTCG1210330.297907240586095No Hit
AGGATTCTCGATGCAAGAAGCTTTGAGCAGTTACAGAAAACCTACAGTAC1111500.2735815008398078No Hit
AGGATTCTCGATGCAAGAAGCTTTGGAAGTTTCGGTTGTTGCATCTCTAC966230.2378251493985133No Hit
AGGATTCTCGATGCAAGAAGCTTACAACATCCAGGATTCCCAGGCCGTCT892100.21957899855977742No Hit
AGGATTCTCGATGCAAGAAGCTTCACCCTTAGAATAAGCTCATTTAGTAT755190.18588035413334636No Hit
AGGATTCTCGATGCAAGAAGCTTTTGCCCTTTTGCTCTACGAGAGGTTTC655680.16138724108919947No Hit
AGGATTCTCGATGCAAGAAGCTTGGAATTTGCAACAAAGTTTCTGAAAGA609250.14995909076621947No Hit
AGGATTCTCGATGCAAGAAGCTTTTGTTCCAACTAGTCTTTCCAAAGTAC524100.12900050795334528No Hit
AGGATTCTCGATGCAAGAAGCTTGCTTTTAGTCCCCGCTTGTCGAAAGGG486240.11968175345780312No Hit
AGGATTCTCGATGCAAGAAGCTTGCTTGATCTTGACTTTCAGTACAAGGC457550.1126200771113397No Hit
AGGATTCTCGATGCAAGAAGCTTCTGGCACCTATTGGGATGTTGTTGGTA450260.1108257369034025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTCT40341400.069.814112
AGGATTC40356950.069.80271
GATTCTC40353550.069.786583
CTCGATG40374900.069.762087
ATTCTCG40323550.069.74664
TCGATGC40376400.069.7318348
CGATGCA40265300.069.730079
ATGCAAG40317100.069.6960711
GATGCAA40284700.069.6912310
TCTCGAT40354150.069.681316
TGCAAGA40311350.069.6071312
GCAAGAA40291100.069.5796213
TTCTCGA40424750.069.560435
GAAGCTT40330150.069.5289117
ACCCAGG5165700.069.5030246
AAGAAGC40318600.069.49802415
CAAGAAG40305850.069.4919714
CTGGACC5378150.069.48460442
CCCAGGA5148900.069.4823747
CACCCAG5195600.069.4404245
Produced by FastQC (version 0.11.2)