FastQCFastQC Report
Tue 9 Jan 2018
HMYHGBBXX_l04n01_dj03.351000000b72e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMYHGBBXX_l04n01_dj03.351000000b72e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8893992
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATT630650670.907484513141No Hit
AAGCTTGCGAAGACAACCTTACCACTTTCGATCGGAAGAGCACACGTCTG91025610.234504371040586Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AAGCTTGCGAAGACAACGTTTTTCTCCAACCTTACCACTTTCGATCGGAA1407291.582292855671559No Hit
AAGCTTGCGAAGACAACGTTTTTCTTCTCCAACCTTACCACTTTCGATCG652650.7338099696963973No Hit
AAGCTTGCGAAGACCACGTTTTTTCACCATTCTGAAGCTGACAGAGTATT510950.5744889359018986No Hit
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTTACAGAGTATT242570.2727346730242168No Hit
AAGCTTGCGAAGACAACGTTTTTTCTTCTCCAACCTTACCACTTTCGATC212710.23916144741304018No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC152450.1714078447563254TruSeq Adapter, Index 22 (98% over 50bp)
AAGCTTGCGAAGACAACGTTTTTTCACGATTCTGAAGCTGACAGAGTATT133340.1499214301069756No Hit
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTAACAGAGTATT123260.1385879366655603No Hit
AAGCTTGCGAAGACAACGTTTTTACACTCTTCTCCAACCTTACCACTTTC109670.12330795890079504No Hit
AAGCTTGCGAAGACAACGTTTTCTTATTCTTCTCCAACCTTACCACTTTC108760.12228479629844506No Hit
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGAATT106530.11977748574543354No Hit
AGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATTA105870.11903541177010278No Hit
AAGCTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTGACAGAGTATTA102870.11566234824587204No Hit
AAGCTTGCGAAGACAACGTTTTTTCACCATTCTGAAGCTTTCCGTTATTT96780.10881502929168364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGA500.070.0049650
GGCCCTC700.070.0041728
GGGCCCG950.070.0041728
CCATGCG150.002225021470.0029948
GACCTAT150.002225319270.0006327
ACGCCCC900.070.00062627
CGGCCTA2050.070.00062627
GGCCCAG900.070.00062627
ACGGCCG900.070.00062625
CGGGCCC950.070.00062627
GGGCCCT650.070.00062627
AGGGCCC650.070.00062626
AAGCTTG8590900.069.999261
ATAGCCG207.9334044E-569.996724
CGGTCCC750.069.996724
TATAGGG700.069.9872523
TATTCGC401.364242E-1069.9872423
TATCGGC401.364242E-1069.9872423
CGGATGC150.002227056569.98685544
CACCCAG3500.069.98685545
Produced by FastQC (version 0.11.2)