FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2814312
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94010.3340425652877151No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT91880.3264741080590922No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC49380.17546029011708725No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG43230.15360770234430296No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA42630.15147574256159232No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA41010.14571945114827356No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA36850.13093786332147964No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC34210.12155724027755274No Hit
CCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGG32770.11644053679904715No Hit
GCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAG31840.11313599913584564No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG31300.11121723533140604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG750.00199199923.0034481
CCGCGCG2700.021.72161962
GCAAGGG16100.020.1458761
CAAGGGC16950.019.1345922
CGCGCGA3051.8189894E-1218.09817965
GGATAAC41750.017.2732471
ACCGCGC3601.8189894E-1216.29121461
GCGCGAT3651.8189894E-1216.06833366
CCCCCCG2654.179492E-815.62220861
AGGGGGG1605.7551934E-415.0960121
CGCGTAT3554.6929927E-1014.5796511
CTAGTTG6650.014.5269438
CGTGGCC22450.014.137691560
GCGCCGT27850.014.12194168
TAACATG49900.014.1060714
GATAACA51000.014.0042932
ATAACAT51750.014.0016013
CGTTCAC22300.013.92362666
GGGCGCC28050.013.6522766
GTTCACG23150.013.56141967
Produced by FastQC (version 0.11.2)