FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4544682
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT111120.24450555616432568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85550.18824199360923383No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC60700.1335627003165458No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA58140.12792974293910991No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA47470.10445175261987527No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG46250.101767296369691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG23450.019.5680541
CAAGGGC23400.019.4613512
CGCGTAT4850.017.7843131
GGGGCAT5100.016.2360321
GGATAAC54100.016.070971
GCGAGGA32400.014.4832547
ACCCGCG5250.013.79956313
GGGCGCC32350.013.22383966
AGGGCGC32750.013.16752365
AGCGTCG33550.013.16362110
GCGTCGT33650.012.81647411
GCGCCGT34750.012.80707368
GATAACA68100.012.6650772
TGGCCGC34250.012.59098220
AAGGGCG38400.012.4885733
GCCCGAT3902.7330316E-812.3851791
CAGCGTC36900.012.3428399
ATAACAT70250.012.2287573
ACATGGC68650.012.213716
AGTGGCC35600.012.21042318
Produced by FastQC (version 0.11.2)