FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4139355
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT135070.32630687631285554No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91950.22213605742923717No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA77940.18829020463333054No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC64310.15536236925801242No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG56980.13765429638192425No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA56960.13760597967557747No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC50220.12132324963671877No Hit
GCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAG43060.10402586876457806No Hit
CCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGG41830.1010543913242522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG28800.024.08211
CAAGGGC30050.022.6199472
GCGAGGA41350.016.438247
GGATAAC69300.016.0329511
CGCGTAT7000.014.7881561
CGAGGAG49250.014.0114498
CGTATTA7950.013.888753
ATAGGGG4253.45608E-1113.79944458
GATAACA80650.013.6903782
AGGGGCG29150.013.49206435
TAACATG82950.013.2280644
GCGCGTT32300.013.13755935
AAGGGCG52700.013.0948253
ATAACAT83150.013.0715963
GGGCGGC30750.013.01473937
GGGGCGG30300.012.97967636
CGGCGCG32800.012.93729133
CCGCGCG4801.8189894E-1112.93682361
CGTAGGG30500.012.89487532
ACATGGC85450.012.8825236
Produced by FastQC (version 0.11.2)