FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2388073
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53810.22532812020403062No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT53000.2219362640924293No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA33070.13847985384031394No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC26370.11042376007768606No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA24910.10431004412344179No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGG851.4431147E-424.3549821
GCAAGGG13050.018.2429071
CAAGGGC13250.017.4452822
GGATAAC28450.014.1892371
CGGGCAT2001.9814578E-413.8011551
GCGGGAT3153.4521872E-713.1439581
ACCCGCG3204.1865314E-712.93695713
GTCGCAT2701.3511317E-512.7788481
GCGAGGA18950.012.74513157
GGCCGTT9800.012.6734763
CGTGGCC10400.012.60524160
GGGCGGC11050.012.17647137
AGGGGCG11050.012.17647135
GCCGTTC10250.012.11707264
CGTTCAC10600.012.04295766
TAGGGGC11500.012.034
GGGGAAT7200.011.980171
GCGAACA3551.468763E-611.6619722
GGGGCGG11600.011.59889536
GATAACA35800.011.5642462
Produced by FastQC (version 0.11.2)