FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4978649
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGACTCTAGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGAC166080.3335844724141027No Hit
ATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCC99200.1992508409409862No Hit
CGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCTTCAAGGTGCGCAT97680.19619780386205174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91190.18316213896581182No Hit
AGCAGATCTATCGATTCTAGCATGACTGGTGGACAGCAAATGGGTCGGGATCTGTACGACGATGACGATAAGGAT76230.1531138266626147No Hit
GTCGGGATCTGTACGACGATGACGATAAGGATCCGATGGCCTCCTCCGAGGACGTCATCAAGGAGTTCATGCGCT58560.11762227062000154No Hit
CGGGAATTGGATCCGGTACCGAGCTCGAATTCTTAGGCGCCGGTGGAGTGGCGGCCCTCGGCGCGCTCGTACTGT57600.11569403667541134No Hit
GTCTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCG50650.10173442634738862No Hit
CTTGACCTCGGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCATCTTGATCTCGCCCTTCAGGGCGCCGTC50590.10161391172585173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT34050.041.5454831
ACTCTAG40700.036.536865
GACTCTA40350.035.7411084
CTGACTC39550.035.6782342
CTAGCAG41500.034.0031558
CTCTAGC49050.029.3328426
TGACTCT50900.028.6717033
TAGCAGA51200.027.6959239
TCTATCG60400.023.304116
CTATCGA60300.023.22855617
GATCTAT63100.022.5256314
AGATCTA65800.021.49668313
TATCGAT66550.021.25442118
ATCTATC67350.021.05296715
GCAGATC68100.020.72103711
AGCAGAT68950.020.56587410
TAGCATG73500.019.15077227
TCTAGCA109650.019.1310567
CTAGCAT74650.018.8557526
ATTCTAG74850.018.80517823
Produced by FastQC (version 0.11.2)