FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4874070
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT114330.23456782524666242No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84590.17355105691957645No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA66610.136661968334472No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC53170.10908747720077881No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG26600.019.0687471
CAAGGGC26850.018.5033842
GGATAAC59750.015.0148811
GCGAGGA37800.013.60027
GATAACA75650.011.812012
TAACATG73450.011.7902724
CGTGGCC14450.011.69837660
GGGCAGG19700.011.55827168
AAGGGCG43650.011.5400073
GCGCGTT28050.011.43851635
ATAACAT77800.011.3083183
CGAGGAG49500.011.2917338
CATGGCC76550.011.2228967
ACATGGC78550.011.1138686
GGCCGTT14750.010.99300863
GGGGAAT17400.010.9068641
CACGAGT77700.010.833878568
GTAGTCC31250.010.81865861
GGCCACG78100.010.77828165
GGCAGGG20950.010.70374169
Produced by FastQC (version 0.11.2)