FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403856
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT45720.32567442814647657No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26580.18933565835812222No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC24260.1728097468686247No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA23570.1678947128480414No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA20900.14887566815969727No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA20500.14602637307530117No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG20170.14367570463067436No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG19730.14054148003783865No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC18890.1345579603606068No Hit
CCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGG16570.1180320488711093No Hit
GCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAG16480.11739095747712017No Hit
ATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCG14580.10385680582623859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG7550.019.1934011
GGATAAC19550.018.1777481
CAAGGGC7950.017.7930682
CGCGGAT1653.840254E-516.7284491
GGGGAAC1704.9591596E-516.2364351
TAGCGCG1350.003238928715.3365957
CGCGCGG1350.003242773515.33386334
TCGCGCG1350.003242773515.33386331
CGCGCAA1605.7514565E-415.09642210
GCGCGCA1400.004125606714.7888589
GCGCGTT12750.014.34167235
TAACATG25400.014.2633214
TCGGCGC12850.014.23006332
CATGCCG13450.014.1108087
CGGCGCG13000.014.06587133
GATAACA25200.013.9647692
ATAGCGC1500.00651751313.803926
AGCGCGC1500.0065205913.8029368
ATGCCGC13750.013.8029368
TGCGCGC1500.006532907513.79900369
Produced by FastQC (version 0.11.2)