FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1955449
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT41660.21304569947873864No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37170.19008422106636377No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA25300.12938204985146634No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC24350.12452383058826899No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA20230.10345450073103415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG10700.023.5394761
CAAGGGC11600.021.1166382
TGCGCGC1200.001477411217.2497769
GCGAGGA17150.015.48998457
CGCGCGA1350.003243865415.3335247
GGGTGTC2152.2652936E-514.44129927
TAGGGTG2454.9621867E-614.08108425
CGAGGAG18500.013.9866588
CGCGTAT1750.001134580113.8012281
GTCGGAA6950.013.4040691
GGGGCAT1600.00999962112.938651
AAGGGCG19200.012.9376583
CGCGGAT2204.4352195E-412.5465711
GCGGGAT1950.00256196612.3857161
CGAATTG3902.7306669E-812.3847683
GGCCCAT2801.9626494E-512.3225251
GGATAAC25500.012.312861
GCCGAAT2306.4477127E-412.0010671
GTCGCAA2651.6061612E-411.7180231
CGCGACC3257.011102E-611.67646927
Produced by FastQC (version 0.11.2)