FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7724289
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT166030.21494534966260323No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG127300.1648048124558778No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA108010.13983164016778762No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC94450.12227662636651736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG41850.019.2101041
CAAGGGC44100.018.2286992
GGATAAC95100.014.3313071
GCGAGGA63700.013.2161697
CGCGCGA4104.0581654E-912.62177547
AGGGCGC58150.012.16218465
GGGCGCC57800.012.11645666
CGTGGCC33150.012.07191860
CGCGTAT8300.012.0556091
GGCCGTT33550.011.7224163
GTCGCCT12250.011.5482731
TAGGGGC43350.011.53985534
CGTTCAC34100.011.53341366
GCGCCGT61150.011.50924268
CCGTTCA35700.011.49968965
CGAGGAG74050.011.4153868
AGGGGCG43250.011.40670435
GCGGGCT3652.0510488E-611.3437761
GATAACA118400.011.3353832
GCCGTTC35150.011.18881564
Produced by FastQC (version 0.11.2)