FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739538
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53180.19412032247773164No Hit
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT49480.1806143955659677No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA35220.12856182319792608No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC30920.11286574597614635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG14500.023.7961861
CAAGGGC15200.022.2450852
GCGAGGA20800.016.7539657
GGCCGTT11100.014.29654163
AGGGGCG13500.014.055325535
TAGGGGC13350.013.95482634
CGTGGCC11500.013.49928660
AAGGGCG25950.013.2962783
GGGGCGG14300.013.26877136
CGTAGGG14200.013.11950332
AGGGCGC18300.012.81922865
TGGCCGT13200.012.80614162
CGAGGAG27500.012.7975568
ACTCGTG14300.012.78650568
CGGTCTG16350.012.65988217
CTGGGTG15050.012.60707821
GCCGTTC12950.012.52080264
CGTTCAC11850.012.51855366
GGGCGCC18500.012.49416166
GGGTGCC15500.012.46362923
Produced by FastQC (version 0.11.2)