FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1663702
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT46920.28202166012903757No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36830.22137377967929353No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA32090.19288310045909665No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC25140.15110879231977842No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA21050.12652506278167605No Hit
CCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGG20810.12508249674521038No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG20020.1203340502085109No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC19540.11744891813557957No Hit
GCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAG19330.11618667285367211No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG17190.10332379236185325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG11850.022.7105711
CAAGGGC11950.022.5191732
GCGAGGA16150.016.6632947
GCGGGCT1400.004129802314.7868291
TGCGCGC1400.004132278314.78549569
CGAGGAG18650.014.6146038
TTGCGCG1850.001726145613.0538668
CGCGTAT2406.36585E-512.9384761
CCTATAA2406.367644E-512.938086521
AAGGGCG21150.012.7235983
GGATAAC24300.012.6367541
GTCACCT14150.012.4355671
AGGGGCG13050.012.4247335
GCCGGTT5003.8198777E-1112.4209361
GGGGCGG13350.012.40431136
ACTCGTG12900.012.30214368
CGACAAT3651.5393744E-712.2885981
GCCAAAT16950.012.2133091
TAGGGGC14250.012.1047234
GGGCGGC13400.012.10056737
Produced by FastQC (version 0.11.2)