FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2755247
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT58930.2138828206690725No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46870.17011179034039414No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA37670.13672095460044054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG16400.018.3042891
CGCGGGG950.00783108618.1603361
CAAGGGC16950.016.6912982
GTCCGCT1756.354468E-515.7735471
CGTGCGC1554.5273473E-415.58047658
TGCCCGC2252.0504613E-615.33316920
CCGTGCG1808.092667E-515.33316757
TACCCTA2959.446012E-915.2040549
CGCCCTA2051.4438721E-515.14672863
GCGTCCC3654.3655746E-1115.12312417
GTGTGCG3756.730261E-1114.7206438
GACGTAC4850.014.22639356
GGGCGCC19650.014.22173866
CGGGCAT3403.9890438E-914.2077921
CGCGTAT3202.7544957E-814.0175091
GCGCCGT20450.013.49667668
CGTGGCC10000.013.45509960
GCGAGGA21150.013.3764717
AGGGCGC21450.013.18914865
AGCGTCG22250.013.18035110
Produced by FastQC (version 0.11.2)