FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1915339
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT56080.2927941215628147No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA38240.19965134109418753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35980.18785186329939504No Hit
CTTGACCTCAGCGTCGTAGTGGCCGCCGTCCTTCAGCTTCAGCCTCTGCTTGATCTCGCCCTTCAGGGCGCCGTC27980.14608380030897924No Hit
GGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGA24640.12864563401048065No Hit
CTCGTCCATGCCGCCGGTGGAGTGGCGGCCCTCGGCGCGTTCGTACTGTTCCACGATGGTGTAGTCCTCGTTGTG24450.12765364251445827No Hit
GATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTC23270.12149285322337194No Hit
CCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAGG22030.1150188034598575No Hit
GCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGAGATCGAG21790.11376576157014501No Hit
CTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGGCCGTTCACGGA19560.10212291401156663No Hit
GTCACCTTCAGCTTGGCGGTCTGGGTGCCCTCGTAGGGGCGGCCCTCGCCCTCGCCCTCGATCTCGAACTCGTGG19560.10212291401156663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG12900.020.3296261
CAAGGGC13100.019.7542922
GGATAAC31300.014.9933991
GTCCGCT1500.00652232213.8027471
CGGGCTC1500.00652570613.8016662
GCGAGGA19950.013.6615217
CGTTCAC9250.013.42654466
CGTGGCC8850.013.2537660
GTCGAAT4701.2732926E-1113.2153951
GCCGCGT2102.991797E-413.14547351
GCGCCGA7800.012.82646265
GATAACA37450.012.806622
CCGTTCA9700.012.44800765
CGAGGAG22100.012.33245858
AACGCGC8200.012.20078162
GTCGGCT2551.1211112E-412.1788941
GTTCACG9350.012.17603367
GCCGTTC10000.012.07456664
GAACGCG8300.012.05378361
ACTCGTG12600.012.04781568
Produced by FastQC (version 0.11.2)