FastQCFastQC Report
Thu 8 Jun 2017
HMY52BGX2_n01_protest_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMY52BGX2_n01_protest_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1386618
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTT30300.21851728450085028No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23020.16601544188810474No Hit
GCAAGGGCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA19070.13752886519574967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGGG7850.018.4590231
CAAGGGC7650.017.5880052
GCCGTTC5100.016.23508364
CGTGGCC5350.015.47587660
GGATAAC17000.015.2209381
GGCCGTT5450.015.19246163
CGTTCAC5950.015.07543466
CCGTTCA6050.014.25601365
GCCTAAT1800.001403639613.4169751
TCGTGGC6550.013.16729359
ACCCGCG2153.6552598E-412.83704213
ACTCGTG8150.012.69922168
AGGGGCG8200.012.62224235
CTGACGC2204.4375894E-412.54529165
CGCTGAC2509.333115E-512.41983963
CGGGTAT1950.00256258512.38491
CGGGACT3357.2821786E-712.3584931
GATAACA20700.012.3331722
TGGCCGT7100.012.14729162
TAGGGGC8550.012.10554234
Produced by FastQC (version 0.11.2)